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Engineering Methods to Produce PHB in the seeds of Camelina

Yield10 PublicationsWe investigated the use of Camelina as a platform for seed-based production of the polymer PHB. Five genetic constructs containing the PHB genes under the control of different combinations of five seed-specific promoters were evaluated for production of polymer in seed plastids using a novel single T1 seed screening method. One construct pMBXS490, containing a combination of the oleosin and glycinin-1 promoters driving the expression of the PHB transgenes, resulted in production of polymer in T1 seeds at levels of up to 15% of the mature seed weight. This PHB level is almost twofold higher than what has previously been reported in a seed.  Our results show that production of high levels of PHB in Camelina oilseeds is feasible with expression of the basic three gene bacterial PHB biosynthetic pathway.

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2015, 2014, 2013, 2012, 2011, 2009, 2008, 2007, 2005, 2004


2015

Malik, M.R., W. Yang, N. Patterson, J. Tang, R.L. Wellinghoff, M.L. Preuss, C. Burkitt, N. Sharma, Y. Ji, J.M. Jez, O.P. Peoples, J.G. Jaworski, E.B. Cahoon, and K.D. Snell, “Production of high levels of poly-3-hydroxybutyrate in plastids of Camelina sativa seeds.” Plant Biotechnology Journal (2015) 13, 675-688.
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Snell, K.D., Signh, V., Brumbley, S.M. “Production of Novel Biopolymers in Plants: Recent Technological Advances and Future Prospects.” Current Opinion in Biotechnology (2015), 32:68-75.
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2014

McQualter, R.B., Petrasovits, L.A., Gebbie, L.K., Schweitzer, D., Blackman, D.M., Chrysanthopoulos P., Hodson, M.P., Plan, M.R., Riches, J.D., Snell, K.D., Brumbley, S.M., and Nielsen L.K. “The Use of an acetoacetyl-CoA Synthase in place of a B-ketothiolase Enhances poly-3-hydroxybutyrate Production in Sugarcane Mesophyll Cells.” Plant Biotechnology Journal (2014) 1-8.
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McQualter, R.B., Somleva, M.N., Gebbie, L.K., Li, X., Petrasovits, L.A., Snell, K.D., Neilsen, L.K. and Brumbley, S.M. “Factors Affecting Polyhydroxybutyrate Accumulation in Mesophyll Cells of Sugarcane and Switchgrass.” BMC Biotechnology (2014) 14:83.
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Somleva, M.N., Xu C.A., Ryan, K.P., Thilmony, R., Peoples, O., Snell, K.D. and Thomson, J. “Transgene Autoexcision in Switchgrass Pollen Mediated by the Bxb1 Recombinase.” BMC Biotechnology (2014) 14:79.
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Mullen, C. A., Boateng, A. A., Schweitzer, D., Sparks, K., and Snell, K. “Mild Pyrolysis of P3HB/Switchgrass Blends for the Production of Bio-oil Enriched with Crotonic Acid.” Journal of Analytical and Applied Pyrolysis, (2014) 107, 40-45.
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2013

Somleva, M.N., Peoples, O.P., and Snell, K.D., "PHA Bioplastics, Biochemicals, and Energy from Crops." Plant Biotechnology Journal (2013), pp. 1-20.

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2012

Petrasovits, L.A., Zhao, L., McQualter, R.B., Snell, K.D., Somleva, M.N., Patterson, N.A., Nielsen, L.K. and Brumbley, S.M., “Enhanced polyhydroxybutyrate production in transgenic sugarcane.” Plant Biotechnology Journal (2012), 10,  pp. 569-578.

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2011

Bohmert-Tatarev, K., McAvoy, S., Daughtry, S., Peoples, O.P., and Snell, K.D., "High levels of bioplastic are produced in fertile transplastomic tobacco plants engineered with a synthetic operon for production of polyhydroxybutyrate." Plant Physiology (2011), 155(4), pp. 1690-1708.

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2009

Snell, K.D., and Peoples, O.P., “PHA bioplastic: A value-added coproduct for biomass biorefineries.”  Biofuels, Bioproducts & Biorefining (2009) 3, pp. 456-467.

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2008

Somleva, M.N., Snell, K.D., Beaulieu, J.J., Peoples, O.P., Garrison, B.R., and Patterson, N.A., "Production of polyhydroxybutyrate in switchgrass,a value-added co-product in an important lignocellulosic biomass crop." Plant Biotechnology Journal (2008) 6, pp. 663–678.

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2007

Kourtz, L., Dillon, K., Daughtry, S., Peoples, O.P., and Snell, K.D., “Chemically inducible expression of the PHB biosynthetic pathway in Arabidopsi.” Transgenic Research, Springer (Netherlands), pp. 759-769.

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2005

Kourtz, L., Dillon, K., Daughtry, S., Madison, L.L., Peoples, O.P., and Snell, K.D., "A Novel Thiolase-Reductase Gene Fusion Promotes the Production of Polyhydroxybutyrate in Arabidopsis." Plant Biotechnology Journal (2005), pp. 435-447.

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2004

Bohmert, K., Peoples, O. P., and Snell, K. D., “Metabolic Engineering: Plastids as Bioreactors.” In Molecular Biology and Biotechnology of Plant Organelles, H. Daniell and C. D. Chase , Kluwer Academic Publishers (Netherlands), 2004: pp. 559-585.

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2002

Skraly, F., "Bioplastics." In Encyclopedia of Environmental Microbiology (2002), J. Wiley & Sons.

Snell, K.D. and Peoples, O.P., "Polyhydroxyalkanoate Polymers and Their Production in Transgenic Plants." Metabolic Engineering (2002), 4, pp. 29-40.

Martin, D.P., Williams, S.F., and Skraly, F.A., "Polymers Biosynthesized by Microorganisms: Polyhydroxyalkanoates." In Methods of Tissue Engineering (2002), 48, Academic Press.

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